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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
03/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, K. C. de; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R.; BEZERRA JUNIOR, R. Q.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
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Afiliação: |
Kelma Costa de Souza, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE; ALICE ANDRIOLI, CNPC; LUCIA HELENA SIDER, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; Rosivaldo Quirino Bezerra Junior; Renato Mesquita Peixoto; Maria Fátima da Silva Teixeira. |
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Título: |
Detecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 43, n. 1266, p. 1-6, 2015. |
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Idioma: |
Português |
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Conteúdo: |
[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium. Menos[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene ... Mostrar Tudo |
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Palavras-Chave: |
Artrite-encefalite Caprina; CAEV; Caprine arthritis encephalit virus; DNA sequence; PCR. |
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Thesagro: |
Caprino; Doença animal; Saliva; Transmissão de doença; Vírus. |
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Thesaurus Nal: |
Animal diseases; Disease transmission; Goats. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136302/1/cnpc-2015-Deteccao.pdf
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Marc: |
LEADER 04687naa a2200349 a 4500
001 2032574
005 2019-04-03
008 2015 bl uuuu u00u1 u #d
100 1 $aSOUZA, K. C. de
245 $aDetecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva.$h[electronic resource]
260 $c2015
520 $a[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium.
650 $aAnimal diseases
650 $aDisease transmission
650 $aGoats
650 $aCaprino
650 $aDoença animal
650 $aSaliva
650 $aTransmissão de doença
650 $aVírus
653 $aArtrite-encefalite Caprina
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aDNA sequence
653 $aPCR
700 1 $aANDRIOLI, A.
700 1 $aSIDER, L. H.
700 1 $aPINHEIRO, R. R.
700 1 $aBEZERRA JUNIOR, R. Q.
700 1 $aPEIXOTO, R. M.
700 1 $aTEIXEIRA, M. F. da S.
773 $tActa Scientiae Veterinariae$gv. 43, n. 1266, p. 1-6, 2015.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 19 | |
| 2. |  | SILVA, I. C. V.; SAMPAIO, J. P. M.; HASHIMOTO, J. M.; SILVA, K. J. D. e; ROCHA, M. de M. Avaliação do rendimento em cotilédones de 12 cultivares de feijão-caupi utilizando o processo mecânico de descorticamento. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 204. Na publicação: KAESEL JACKSON DAMASCENO-SILVA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
|    |
| 3. | | SILVA, I. C. V.; HASHIMOTO, J. M.; SILVA, K. J. D. e; CARVALHO, C. W. P. de; ASCHERI, J. L. R.; GALDEANO, M. C. Avaliação da qualidade tecnológica de produtos extrusados obtidos a partir da farinha de coproduto de feijão-caupi. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 3., 2017, Teresina, PI. Anais da III Jornada Científica da Embrapa Meio-Norte. Teresina: Embrapa Meio-Norte, 2017. p. 28.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | GOMES, F. O.; SILVA, I. C. V.; FRANCO, L. J. D.; HASHIMOTO, J. M.; SILVA, K. J. D. e; ROCHA, M. de M. Chemical and mineral composition of the raw and cooked immature grains of cowpea genotypes. Revista Caatinga, Mossoró, v. 37, e11727, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 6. | | GOMES, F. de O.; FREITAS, T. K. T.; RODRIGUES, L. L.; SILVA, I. C. V.; SILVA, K. J. D. e; ROCHA, M. de M. Composição centesimal e valor energético total nos grãos imaturos de linhagens e cultivares de feijão-caupi. In: JORNADA CIENTIFICA DA EMBRAPA MEIO-NORTE, 6., 2020,Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2021. p. 29 (Embrapa Meio-Norte. Documentos, 284).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 7. | | SILVA, I. C. V.; HASHIMOTO, J. M.; SILVA, K. J. D. e; CARVALHO, C. W. P. de; ASCHERI, J. L. R.; GALDEANO, M. C. Efeito dos parâmetros de extrusão sobre as propriedades tecnológicas de extrusado de feijão-caupi. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 5., 2019, Fortaleza. Sustentabilidade e inovações tecnológicas para o feijão-caupi: desafios e perspectivas: anais. Fortaleza: Universidade Federal do Ceará: Embrapa Meio-Norte, 2019. 5 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Meio-Norte. |
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| 8. | | SILVA, I. C. V.; SILVA, K. J. D. e; HASHIMOTO, J. M.; ROCHA, M. de M.; CARVALHO, J. L. V. de. Efeito do processo de extrusão na composição nutricional de extrusados de feijão-caupi BRS Tumucumaque. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 5., 2019,Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2022. 96 p. (Embrapa Meio-Norte. Documentos, 285). p. 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
| |
| 9. | | SILVA, I. C. V.; SILVA, K. J. D. e; HASHIMOTO, J. M.; ROCHA, M. de M.; CARVALHO, J. L. V. de. Efeito do processo de extrusão na composição nutricional de extrusados de feijão-caupi BRS Tumucumaque. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 5., 2019,Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2022. 96 p. (Embrapa Meio-Norte. Documentos, 285). p. 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 10. | | SILVA, I. C. V.; SILVA, K. J. D. e; HASHIMOTO, J. M.; CARVALHO, C. W. P. de; ASCHERI, J. L. R.; GALDEANO, M. C.; ROCHA, M. de M. Effect of different processing conditions to obtain expanded extruded based on cowpea. Brazilian Journal of Food Technology, v. 26, e2022052, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Meio-Norte. |
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| 11. | | GOMES, F. de O.; SILVA, I. C. V.; FREITAS, T. K. T.; SILVA, D. J. S.; RODRIGUES, L. L.; SABÓIA, R. C. B.; ROCHA, M. de M. Determination of minerals in immature grains of cowpea lineages and cultivars. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 14., 2021, Campinas. Anais eletrônicos... Campinas: Galoá, 2021. SLACA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 12. | | CARNIB, L. P. de A.; SILVA, I. C. V.; LUGO, L. M. N.; FRANCO, L. J. D.; SILVA, K. J. D. e; ROCHA, M. de M. Perfil de minerais na vagem verde de genótipos de feijão-de-metro (Vigna unguiculata ssp. sesquipedalis) para o consumo como salada. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 3., 2017, Teresina, PI. Anais da III Jornada Científica da Embrapa Meio-Norte. Teresina: Embrapa Meio-Norte, 2017. p. 69.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 13. | | FREITAS, T. K. T.; GOMES, F. de O.; SILVA, I. C. V.; ARAÚJO, M. dos S.; SILVA, K. J. D. e; ROCHA, M. de M. Variabilidade genética de linhagens elite de feijão-caupi quanto ao teor de zinco no grão. In: JORNADA CIENTIFICA DA EMBRAPA MEIO-NORTE, 6., 2020,Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2021. p. 67 (Embrapa Meio-Norte. Documentos, 284).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 14. | | COSTA, N. Q.; SAMPAIO, J. P. M.; SILVA, I. C. V.; CARNIB, L. P. de A.; MOREIRA-ARAÚJO, R. S. dos R.; HASHIMOTO, J. M.; SILVA, K. J. D. e; ROCHA, M. de M. Caracterização nutricional de grãos verdes crus e após processamento térmico em cultivares de feijão-caupi. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 143. Na publicação: Kaesel Jackson Damasceno-Silva.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 15. | | RIOS, M. J. B. L.; SAMPAIO, J. P. M.; LUGO, L. M. N.; SILVA, I. C. V.; ARAÚJO, R. S. dos R. M.; HASHIMOTO, J. M.; SILVA, K. J. D. e; ROCHA, M. de M. Composição química e avaliação microbiológica de farinhas de cinco cultivares comerciais de feijão-caupi [Vigna unguiculata (L.) Walp.]. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 211. Na publicação: KAESEL JACKSON DAMASCENO-SILVA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 16. | | BARBOSA, C. Z. de M. C. D.; SILVA, I. C. V.; CARNIB, L. P. de A.; OLIVEIRA, D. S. V. de; MOREIRA-ARAÚJO, R. S. dos R.; HASHIMOTO, J. M.; SILVA, K. J. D. e; ROCHA, M. de M. Correlações entre caracteres nutricionais e o tempo de cocção em feijão-caupi. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 214. Na publicação: KAESEL JACKSON DAMASCENO-SILVA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 17. | | RIOS, M. J. B. L.; LUGO, L. M. N.; SAMPAIO, J. P. M.; SILVA, I. C. V.; ARAÚJO, R. S. dos R. M.; HASHIMOTO, J. M.; SILVA, K. J. D. e; ROCHA, M. de M. Parâmetros físicos de farinhas de cinco cultivares de feijão-caupi [Vigna unguiculata (L.) Walp.]. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercados: resumos. Brasília, DF: Embrapa, 2016. p. 227.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 18. | | FREITAS, T. K. T.; GOMES, F. de O.; ARAÚJO, M. dos S.; SILVA, I. C. V.; SILVA, D. J. S.; SILVA, K. J. D. e; ROCHA, M. de M. Potential of cowpea genotypes for nutrient biofortification and cooking quality. Revista Ciência Agronômica, v. 53, e20218048, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 19. | | BRITO, M. F. F. de; CARNIB, L. P. de A.; SILVA, I. C. V.; LUGO, L. M. N.; HASHIMOTO, J. M.; SILVA, K. J. D. e; ROCHA, M. de M. Influência do tempo de embebição e tipo de recipiente no tempo de cocção do feijão-caupi. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 178. Na publicação: KAESEL JACKSON DAMASCENO-SILVA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 19 | |
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